Functional analysis of antigen-specific T lymphocytes by serial measurement of gene expression in peripheral blood mononuclear cells and tumor specimens.
Published
Journal Article
The cloning of cancer Ags recognized by T cells has provided potentially new tools to enhance immunity against metastatic cancer. The biological monitoring of effective immunization has, however, remained a dilemma. We describe here a sensitive molecular quantitation methodology that allows analysis of in vivo immune response to vaccination. Metastatic melanoma patients were immunized with a synthetically modified peptide epitope (209-2M) from the melanoma self-Ag gp100. Using serial gene expression analysis, we report functional evidence of vaccine-induced CTL reactivity in fresh cells obtained directly from the peripheral blood of postimmunized patients. Further, we demonstrate in vivo localization of vaccine-induced immune response within the tumor microenvironment. The results of these molecular assays provide direct evidence that peptide immunization in humans can result in tumor-specific CTL that localize to metastatic sites.
Full Text
Duke Authors
Cited Authors
- Kammula, US; Lee, KH; Riker, AI; Wang, E; Ohnmacht, GA; Rosenberg, SA; Marincola, FM
Published Date
- December 15, 1999
Published In
Volume / Issue
- 163 / 12
Start / End Page
- 6867 - 6875
PubMed ID
- 10586088
Pubmed Central ID
- 10586088
International Standard Serial Number (ISSN)
- 0022-1767
Language
- eng
Conference Location
- United States