Functional analysis of antigen-specific T lymphocytes by serial measurement of gene expression in peripheral blood mononuclear cells and tumor specimens.

Journal Article

The cloning of cancer Ags recognized by T cells has provided potentially new tools to enhance immunity against metastatic cancer. The biological monitoring of effective immunization has, however, remained a dilemma. We describe here a sensitive molecular quantitation methodology that allows analysis of in vivo immune response to vaccination. Metastatic melanoma patients were immunized with a synthetically modified peptide epitope (209-2M) from the melanoma self-Ag gp100. Using serial gene expression analysis, we report functional evidence of vaccine-induced CTL reactivity in fresh cells obtained directly from the peripheral blood of postimmunized patients. Further, we demonstrate in vivo localization of vaccine-induced immune response within the tumor microenvironment. The results of these molecular assays provide direct evidence that peptide immunization in humans can result in tumor-specific CTL that localize to metastatic sites.

Full Text

Duke Authors

Cited Authors

  • Kammula, US; Lee, KH; Riker, AI; Wang, E; Ohnmacht, GA; Rosenberg, SA; Marincola, FM

Published Date

  • December 15, 1999

Published In

Volume / Issue

  • 163 / 12

Start / End Page

  • 6867 - 6875

PubMed ID

  • 10586088

International Standard Serial Number (ISSN)

  • 0022-1767

Language

  • eng

Conference Location

  • United States