Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane.

Journal Article (Journal Article)

A highly catalytic activity microperoxidase-11 (MP-11) biosensor for H(2)O(2) was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (DDAB) lipid membrane. The enzyme electrode thus obtained responded to H(2)O(2) without electron mediator or promoter, at a potential of +0.10 V versus Agmid R:AgCl. A linear calibration curve is obtained over the range from 2.0 x 10(-5) to 2.4 x 10(-3) M. The biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8 x 10(-7) M (S/N=3) Providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors.

Full Text

Duke Authors

Cited Authors

  • Huang, W; Jia, J; Zhang, Z; Han, X; Tang, J; Wang, J; Dong, S; Wang, E

Published Date

  • September 2003

Published In

Volume / Issue

  • 18 / 10

Start / End Page

  • 1225 - 1230

PubMed ID

  • 12835040

International Standard Serial Number (ISSN)

  • 0956-5663

Digital Object Identifier (DOI)

  • 10.1016/s0956-5663(03)00070-8


  • eng

Conference Location

  • England