Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice.

Published

Journal Article

We developed chimeric Sindbis (SINV)/eastern equine encephalitis (EEEV) viruses and investigated their potential for use as live virus vaccines against EEEV. One vaccine candidate contained structural protein genes from a typical North American EEEV strain, while the other had structural proteins from a naturally attenuated Brazilian isolate. Both chimeric viruses replicated efficiently in mammalian and mosquito cell cultures and were highly attenuated in mice. Vaccinated mice did not develop detectable disease or viremia, but developed high titers of neutralizing antibodies. Upon challenge with EEEV, mice vaccinated with >10(4) PFU of the chimeric viruses were completely protected from disease. These findings support the potential use of these SIN/EEEV chimeras as safe and effective vaccines.

Full Text

Duke Authors

Cited Authors

  • Wang, E; Petrakova, O; Adams, AP; Aguilar, PV; Kang, W; Paessler, S; Volk, SM; Frolov, I; Weaver, SC

Published Date

  • October 23, 2007

Published In

Volume / Issue

  • 25 / 43

Start / End Page

  • 7573 - 7581

PubMed ID

  • 17904699

Pubmed Central ID

  • 17904699

International Standard Serial Number (ISSN)

  • 0264-410X

Digital Object Identifier (DOI)

  • 10.1016/j.vaccine.2007.07.061

Language

  • eng

Conference Location

  • Netherlands