Determination of sulfadiazine and sulfamethoxazole by capillary electrophoresis with end-column electrochemical detection.

Journal Article (Journal Article)

Capillary electrophoresis (CE) with end-column electrochemical detection (EC) of sulfadiazine (SDZ) and sulfamethoxazole (SMZ) is described. Under the optimum conditions, SDZ and SMZ were separated satisfactorily, and a highly sensitive and stable response was obtained at a potential of 1.1 V versus Ag/AgCl. Optimized end-column detection provides detection limits as low as 0.1 microM for both compounds, which corresponds to 0.024 and 0.021 fmol with peak efficiencies of 394,000 and 335,000 theoretical plates for SDZ and SMZ, respectively. The calibration graph was linear over three order of magnitude. The relative standard deviations (n = 12) of peak currents and migration times were 2.3 and 2.7%, and 0.8 and 1.3%, respectively, for the two compounds. The proposed method was applied to the analysis of tablets and human urine samples with satisfactory results.

Full Text

Duke Authors

Cited Authors

  • You, T; Yang, X; Wang, E

Published Date

  • November 1998

Published In

Volume / Issue

  • 123 / 11

Start / End Page

  • 2357 - 2360

PubMed ID

  • 10396813

International Standard Serial Number (ISSN)

  • 0003-2654

Digital Object Identifier (DOI)

  • 10.1039/a805488f


  • eng

Conference Location

  • England