Electrochemistry and spectroscopy study on the interaction of microperoxidase-11 with lipid membrane.

Journal Article (Journal Article)

The interaction of microperoxidase-11 (MP11) with cationic lipid vesicles of didodecyldimethylammonium bromide (DDAB) induces an alpha-helical conformation from random coil conformations in solution and this change then makes heme macrocycle more distorted. DDAB-induced MP11 conformations were investigated by cyclic votammetry (CV), circular dichroism (CD) and UV-vis spectrometry. All results indicate that the binding of MP11 in solution to DDAB vesicles and the ordered structure formation are driven by mostly electrostatic interaction between negatively charged residues in the undecapeptide and positively charged lipid headgroups on the membrane surface. Upon binding to DDAB, its half-peak potential was also changed. The mechanism of the interaction between MP11 and DDAB was also discussed.

Full Text

Duke Authors

Cited Authors

  • Huang, W; Zhang, Z; Han, X; Tang, J; Peng, Z; Dong, S; Wang, E

Published Date

  • December 11, 2001

Published In

Volume / Issue

  • 94 / 1-2

Start / End Page

  • 165 - 173

PubMed ID

  • 11744197

International Standard Serial Number (ISSN)

  • 0301-4622

Digital Object Identifier (DOI)

  • 10.1016/s0301-4622(01)00230-7


  • eng

Conference Location

  • Netherlands