High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines.

Published

Journal Article

Tumours develop from clonally expanded population of cells harbouring aberrations of oncogenes and tumour suppressor genes. In this study, metaphase and array comparative genomic hybridization showed good correlation of aberration profiles in lung adenocarcinoma cell lines from patients with different tobacco exposure. Recurrent DNA gains were found at chromosomes 1, 7, 8, 17, 20, and deletions at 1, 3, 8, 9, 10, 12, 17, 18, 19. Candidate tumour loci and encompassed genes at 7p21 (AGR2), 8q21(TPD52), 20q13 (ZNF217, WFDC2, EEF1A2) and 10p15 (KLF6) were analyzed by dual colour FISH for genomic changes and quantitative PCR for expression changes. Results indicated that EEF1A2 and KLF6 were strong candidates of oncogene and tumour suppressor genes, respectively. This study illustrates, a practical strategy for identifying candidate cancer genes from microarray data.

Full Text

Duke Authors

Cited Authors

  • Zhu, H; Lam, DCL; Han, KC; Tin, VPC; Suen, WS; Wang, E; Lam, WK; Cai, WW; Chung, LP; Wong, MP

Published Date

  • January 8, 2007

Published In

Volume / Issue

  • 245 / 1-2

Start / End Page

  • 303 - 314

PubMed ID

  • 16517066

Pubmed Central ID

  • 16517066

International Standard Serial Number (ISSN)

  • 0304-3835

Digital Object Identifier (DOI)

  • 10.1016/j.canlet.2006.01.020

Language

  • eng

Conference Location

  • Ireland