Determination of ranitidine in urine by capillary electrophoresis-electrochemiluminescent detection.

Journal Article (Journal Article)

The fast analysis of ranitidine is of clinical importance in understanding its efficiency and a patient's treatment history. In this paper, a novel determination method for ranitidine based on capillary electrophoresis-electrochemiluminescence detection is described. The conditions affecting separation and detection were investigated in detail. End-column detection of ranitidine in 5 mM Ru(bpy)(3)(2+) solution at applied voltage of 1.20 V was performed. Favorable ECL intensity with higher column efficiency was achieved by electrokinetic injection for 10s at 10 kV. The R.S.D. values of ECL intensity and migration time were 6.38 and 1.84% for 10(-4) M and 6.01 and 0.60% for 10(-5) M, respectively. A detection limit of 7 x 10(-8) M (S/N=3) was achieved. The proposed method was applied satisfactorily to the determination of ranitidine in urine in 6 min.

Full Text

Duke Authors

Cited Authors

  • Gao, Y; Tian, Y; Sun, X; Yin, X-B; Xiang, Q; Ma, G; Wang, E

Published Date

  • March 7, 2006

Published In

Volume / Issue

  • 832 / 2

Start / End Page

  • 236 - 240

PubMed ID

  • 16442350

International Standard Serial Number (ISSN)

  • 1570-0232

Digital Object Identifier (DOI)

  • 10.1016/j.jchromb.2006.01.004


  • eng

Conference Location

  • Netherlands