Assembly of alternating polycation and DNA multilayer films by electrostatic layer-by-layer adsorption.

Journal Article (Journal Article)

The assembly of alternating DNA and positively charged poly(dimethyldiallylammonium chloride) (PDDA) multilayer films by electrostatic layer-by-layer adsorption has been studied. The real-time surface plasmon resonance (BIAcore) technique was used to characterize and monitor the formation of multilayer films in solution in real time continuously. Electrochemical impedance spectroscopy (EIS) and UV-vis absorbance measurements were also used to study the film assembly, and linear film growth was observed. All the results indicate that the uniform multilayer can be obtained on the poly(ethylenimine)- (PEI-) coated substrate surface. The kinetics of the adsorption of DNA on PDDA surface was also studied by the real-time BIAcore technique; the observed rate constant was calculated using a Langmuir model (k(obs) = (1.28 +/- 0.08) x 10(-2) s(-1).

Full Text

Duke Authors

Cited Authors

  • Pei, R; Cui, X; Yang, X; Wang, E

Published Date

  • 2001

Published In

Volume / Issue

  • 2 / 2

Start / End Page

  • 463 - 468

PubMed ID

  • 11749207

International Standard Serial Number (ISSN)

  • 1525-7797

Digital Object Identifier (DOI)

  • 10.1021/bm0001289


  • eng

Conference Location

  • United States