Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1alpha and eEF1A-2/S1 in the central nervous system during mouse development.


Journal Article

During early postnatal development, a switch occurs between eEF1A-1/EF-1alpha and eEF1A-2/S1, homologous peptide elongation factors, in brain, heart, and skeletal muscle; eEF1A-2/S1 becomes the major form expressed in maturity. By immunofluorescent labeling, we detected both homologues in the developing brains of wild-type and wasted mutant mice, carrying a deletion in the eEF1A-2/S1 gene; we found that brain expression of eEF1A-2/S1 protein is restricted to mature, terminally differentiated neurons, and coincides with the disappearance of eEF1A-1/EF-1alpha 20 days after birth. Furthermore, no elongation factor 1A is present in wasted mutant mice neurons following the developmental switch, indicating that the genetic regulation silencing eEF1A-1/EF-1alpha is still functional.

Full Text

Duke Authors

Cited Authors

  • Pan, J; Ruest, L-B; Xu, S; Wang, E

Published Date

  • March 22, 2004

Published In

Volume / Issue

  • 149 / 1

Start / End Page

  • 1 - 8

PubMed ID

  • 15013623

Pubmed Central ID

  • 15013623

International Standard Serial Number (ISSN)

  • 0165-3806

Digital Object Identifier (DOI)

  • 10.1016/j.devbrainres.2003.10.011


  • eng

Conference Location

  • Netherlands