Purification of alpha-sarcin and an antifungal protein from Aspergillus giganteus by blue sepharose CL-6B affinity chromatography.

Published

Journal Article

A simple method for preparation of alpha-sarcin and an antifungal protein (AFP) from the mold Aspergillus giganteus MDH 18894 has been developed. alpha-Sarcin and AFP were purified simultaneously by carboxymethylcellulose 52 cation-exchange chromatography and Blue Sepharose CL-6B affinity chromatography. By this method, 4.2 mg of alpha-sarcin and 6.8 mg of AFP were obtained from 2 liters of medium. Compared with other purification methods such as gel-filtration chromatography, this procedure was simple and specific. The purified alpha-sarcin and AFP were homogeneous characterized on SDS-polyacrylamide gel. The enzymatic activity of several ribosome-inactivating proteins such as alpha-sarcin, trichosanthin, and cinnamomin was significantly inhibited by the dye Cibacron blue F3GA. In 50 microliter of reaction mixture, 10 microM of the dye could inhibit 50% activity of cinnamomin (7 x 10(-9) M), whereas 50% inhibition of the enzymatic activity of trichosanthin (7 x 10(-9) M) and alpha-sarcin (1 x 10(-7) M) required 100 and 50 microM of the dye, respectively.

Full Text

Duke Authors

Cited Authors

  • Hao, JJ; Xu, YZ; Geng, CD; Liu, WY; Wang, ED; Gong, ZZ; Ulbrich, N

Published Date

  • November 1998

Published In

Volume / Issue

  • 14 / 2

Start / End Page

  • 295 - 301

PubMed ID

  • 9790894

Pubmed Central ID

  • 9790894

International Standard Serial Number (ISSN)

  • 1046-5928

Language

  • eng

Conference Location

  • United States