Transcriptional profiling of Alzheimer blood mononuclear cells by microarray.

Published

Journal Article

We evaluated pathomechanisms and systemic manifestations of Alzheimer disease (AD), an aging-related dementing neurodegenerative disorder, by expression profiling. Blood mononuclear cell (BMC) transcriptomes of sporadic AD subjects and aged-matched normal elderly controls (NEC) were compared using the human NIA microarray. Relative to the NEC samples, the Alzheimer BMC exhibited a significant decline in the expression of genes concerned with cytoskeletal maintenance, cellular trafficking, cellular stress response, redox homeostasis, transcription and DNA repair. We observed decreased expression of several genes which may impact amyloid-beta production and the processing of the microtubule-associated protein tau. The microarray results were validated by quantitative real time PCR and revealed gender differences in the levels of altered gene expression. Our findings attest to the systemic nature of gene dys-regulation in sporadic AD, implicate disruption of cytoskeletal integrity, DNA repair mechanisms and cellular defenses in this condition, and suggest novel pathways of beta-amyloid deposition in this disease. BMC are highly accessible and may reflect molecular events germane to the neuropathophysiology of AD.

Full Text

Duke Authors

Cited Authors

  • Maes, OC; Xu, S; Yu, B; Chertkow, HM; Wang, E; Schipper, HM

Published Date

  • December 2007

Published In

Volume / Issue

  • 28 / 12

Start / End Page

  • 1795 - 1809

PubMed ID

  • 16979800

Pubmed Central ID

  • 16979800

Electronic International Standard Serial Number (EISSN)

  • 1558-1497

Digital Object Identifier (DOI)

  • 10.1016/j.neurobiolaging.2006.08.004

Language

  • eng

Conference Location

  • United States