Quiescent phenotype of tumor-specific CD8+ T cells following immunization.

Published

Journal Article

In a human melanoma model of tumor antigen (TA)-based immunization, we tested the functional status of TA-specific CD8+ cytotoxic T lymphocytes. A "quiescent" phenotype lacking direct ex vivo cytotoxic and proliferative potential was identified that was further characterized by comparing its transcriptional profile to that of TA-specific T cells sensitized in vitro by exposure to the same TA and the T-cell growth factor interleukin 2 (IL-2). Quiescent circulating tumor-specific CD8+ T cells were deficient in expression of genes associated with T-cell activation, proliferation, and effector function. This quiescent status may explain the observed lack of correlation between the presence of circulating immunization-induced lymphocytes and tumor regression. In addition, the activation of TA-specific T cells by in vitro antigen recall and IL-2 suggests that a complete effector phenotype might be reinstated in vivo to fulfill the potential of anticancer vaccine protocols.

Full Text

Duke Authors

Cited Authors

  • Monsurrò, V; Wang, E; Yamano, Y; Migueles, SA; Panelli, MC; Smith, K; Nagorsen, D; Connors, M; Jacobson, S; Marincola, FM

Published Date

  • October 1, 2004

Published In

Volume / Issue

  • 104 / 7

Start / End Page

  • 1970 - 1978

PubMed ID

  • 15187028

Pubmed Central ID

  • 15187028

International Standard Serial Number (ISSN)

  • 0006-4971

Digital Object Identifier (DOI)

  • 10.1182/blood-2004-02-0525

Language

  • eng

Conference Location

  • United States