Prospective molecular profiling of melanoma metastases suggests classifiers of immune responsiveness.

Journal Article

We amplified RNAs from 63 fine needle aspiration (FNA) samples from 37 s.c. melanoma metastases from 25 patients undergoing immunotherapy for hybridization to a 6108-gene human cDNA chip. By prospectively following the history of the lesions, we could correlate transcript patterns with clinical outcome. Cluster analysis revealed a tight relationship among autologous synchronously sampled tumors compared with unrelated lesions (average Pearson's r = 0.83 and 0.7, respectively, P < 0.0003). As reported previously, two subgroups of metastatic melanoma lesions were identified that, however, had no predictive correlation with clinical outcome. Ranking of gene expression data from pretreatment samples identified approximately 30 genes predictive of clinical response (P < 0.001). Analysis of their annotations denoted that approximately half of them were related to T-cell regulation, suggesting that immune responsiveness might be predetermined by a tumor microenvironment conducive to immune recognition.

Full Text

Duke Authors

Cited Authors

  • Wang, E; Miller, LD; Ohnmacht, GA; Mocellin, S; Perez-Diez, A; Petersen, D; Zhao, Y; Simon, R; Powell, JI; Asaki, E; Alexander, HR; Duray, PH; Herlyn, M; Restifo, NP; Liu, ET; Rosenberg, SA; Marincola, FM

Published Date

  • July 1, 2002

Published In

Volume / Issue

  • 62 / 13

Start / End Page

  • 3581 - 3586

PubMed ID

  • 12097256

International Standard Serial Number (ISSN)

  • 0008-5472

Language

  • eng

Conference Location

  • United States