Amperometric glucose biosensor based on lipid film.

Published

Journal Article

A novel glucose biosensor based on cast lipid film was developed. This model of biological membrane was used to supply a biological environment on the surface of the electrode, moreover it could greatly reduce the interference and effectively exclude hydrophilic electroactive material from reaching the detecting surface. TTF was selected as a mediator because of its high electron-transfer efficiency, and it was incorporated in the lipid film firmly. Glucose oxidase was immobilized in hydrogel covered on the lipid film. The effects of pH, operating potential were explored for the optimum analytical performance by using amperometric method. The response time of the biosensor was less than 20 s, and the linear range is up to 10 mmol l(-1) (corr. coeff. 0.9932) with the detection limit of 2 x 10(-5) mol l(-1). The biosensor also exihibited good stability and reproducibility.

Full Text

Duke Authors

Cited Authors

  • Wu, Z; Wang, B; Dong, S; Wang, E

Published Date

  • June 2000

Published In

Volume / Issue

  • 15 / 3-4

Start / End Page

  • 143 - 147

PubMed ID

  • 11286331

Pubmed Central ID

  • 11286331

International Standard Serial Number (ISSN)

  • 0956-5663

Digital Object Identifier (DOI)

  • 10.1016/s0956-5663(00)00063-4

Language

  • eng

Conference Location

  • England