Drug-human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection.

Published

Journal Article

A new technique for investigating drug-protein binding was developed employing capillary electrophoresis (CE) coupled with tris(2,2'-bipyridyl) ruthenium(II) [Ru(bpy)(3) (2+)] electrochemiluminescence (ECL) (CE-ECL) detection after equilibrium dialysis. Three basic drugs, namely pridinol, procyclidine and its analogue trihexyphenidyl, were successfully separated by capillary zone electrophoresis with end-column Ru(bpy)(3) (2+) ECL detection. The relative drug binding to human serum albumin (HSA) for each single drug as well as for the three drugs binding simultaneously was calculated. It was found that the three antiparkinsonian drugs compete for the same binding site on HSA. This work demonstrated that Ru(bpy)(3) (2+) CE-ECL can be a suitable technique for studying drug-protein binding.

Full Text

Duke Authors

Cited Authors

  • Zhao, X; You, T; Liu, J; Sun, X; Yan, J; Yang, X; Wang, E

Published Date

  • October 2004

Published In

Volume / Issue

  • 25 / 20

Start / End Page

  • 3422 - 3426

PubMed ID

  • 15490448

Pubmed Central ID

  • 15490448

International Standard Serial Number (ISSN)

  • 0173-0835

Digital Object Identifier (DOI)

  • 10.1002/elps.200305930

Language

  • eng

Conference Location

  • Germany