A longitudinal replicate study of immunosuppressive drugs: a College of American Pathologists study.
Journal Article
OBJECTIVE: To identify the sources of analytical variation for cyclosporine and tacrolimus in a 3-year longitudinal study. DESIGN: Two pools of whole blood were spiked with cyclosporine and tacrolimus, respectively. One aliquot of cyclosporine and 2 of the tacrolimus pool were distributed in the first and last mailing for years 1999 to 2001. For both drugs, the total variance for each method was partitioned into within- and between-laboratory components. SETTING: The A and C mailings of the 1999, 2000, and 2001 AACC/CAP [American Association for Clinical Chemistry/College of American Pathologists] Immunosuppressive Drugs (CS) Monitoring Survey. MAIN OUTCOME MEASURES: For each drug, total variance was partitioned into specimen, mailing, year, and interlaboratory effects for each analytical method. PARTICIPANTS: The 292 laboratories for cyclosporine and 177 laboratories for tacrolimus enrolled in the survey from 1999 to 2001. RESULTS: For both cyclosporine and tacrolimus, the major source of imprecision came from within-laboratory factors, which accounted for nearly 85% (range, 77% to 90%) of the total variance. For cyclosporine, the major component of within-laboratory variance was between-mailing, within-year effect, whereas for tacrolimus it was the between-year, within-laboratory variation. CONCLUSION: The major source of long-term survey imprecision for cyclosporine and tacrolimus is within-laboratory factors. The finding that 85% of the total variance was due to within-laboratory variation is similar to other therapeutic drugs.
Full Text
Duke Authors
Cited Authors
- Steele, BW; Wang, E; Soldin, SJ; Klee, G; Elin, RJ; Witte, DL; College of American Pathologists Study,
Published Date
- March 2003
Published In
Volume / Issue
- 127 / 3
Start / End Page
- 283 - 288
PubMed ID
- 12653570
Pubmed Central ID
- 12653570
Electronic International Standard Serial Number (EISSN)
- 1543-2165
Digital Object Identifier (DOI)
- 10.1043/0003-9985(2003)127<0283:ALRSOI>2.0.CO;2
Language
- eng
Conference Location
- United States