Fabrication of a poly(dimethylsiloxane)-based electrochemiluminescence detection cell for capillary electrophoresis.

Journal Article (Journal Article)

An easy but effective technique is described here for quick fabrication of low-cost electrochemiluminescence detection cells for capillary electrophoresis. The technique is based on molding of poly(dimethylsiloxane) (PDMS) with a capillary column inserted into a pipet tip. Two access holes are left in the PDMS slab; they provide neat accommodations for the separation capillary and the working electrode made with the same type of tip. Since the access holes are well-aligned, the electrode and the capillary are automatically aligned; thus, end-column detection is easily obtained. Fabrication of the detection cell is straightforward; no micromechanical operation is included. Also the principle for the procedure makes it possible to efficiently batch production detection cells with good reproducibility. Because of the end-column scheme, the cell can be adopted for electrophoresis with electrochemical detection as well.

Full Text

Duke Authors

Cited Authors

  • Yan, J; Yang, X; Wang, E

Published Date

  • August 15, 2005

Published In

Volume / Issue

  • 77 / 16

Start / End Page

  • 5385 - 5388

PubMed ID

  • 16097784

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac050581g


  • eng

Conference Location

  • United States