Real-time immunoassay of antibody activity in serum by surface plasmon resonance biosensor.

Journal Article (Journal Article)

A surface plasmon resonance biosensor has been used to determine antibody activity in serum. As a model system, the interaction of mouse IgG and sheep anti-mouse IgG polyclonal antibody was investigated in real time. The factors, including pH value, ionic strength, protein concentration, influencing electrostatic adsorption of mouse IgG protein onto carboxylated dextran-coated sensor chip surface, were studied. The procedures of mouse IgG protein immobilization and immune reaction were monitored in real time. The regeneration effect using the different elution reagents was also investigated. The same mouse IgG immobilized surface can be used for 100 cycles of binding and elution with only 0.38% loss per regeneration in reactivity. The results show that the surface plasmon resonance biosensor is a rapid, simple, sensitive, accurate and reliable detection technique for real-time immunoassay of antibody activity. The assay allows antibodies to be detected and studied in their native form without any purification.

Full Text

Duke Authors

Cited Authors

  • Pei, R; Cui, X; Yang, X; Wang, E

Published Date

  • December 4, 2000

Published In

Volume / Issue

  • 53 / 3

Start / End Page

  • 481 - 488

PubMed ID

  • 18968133

Electronic International Standard Serial Number (EISSN)

  • 1873-3573

Digital Object Identifier (DOI)

  • 10.1016/s0039-9140(00)00495-1

Language

  • eng

Conference Location

  • Netherlands