Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals.

Journal Article (Journal Article)

In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.

Full Text

Duke Authors

Cited Authors

  • Lacelle, C; Wang, E

Published Date

  • July 2002

Published In

Volume / Issue

  • 123 / 10

Start / End Page

  • 1415 - 1418

PubMed ID

  • 12297344

International Standard Serial Number (ISSN)

  • 0047-6374

Digital Object Identifier (DOI)

  • 10.1016/s0047-6374(02)00074-x


  • eng

Conference Location

  • Ireland