Capillary electrophoresis with electrochemiluminescence detection for measurement of aspartate aminotransferase and alanine aminotransferase activities in biofluids.

Published

Journal Article

A new sensitive assay for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in biofluids was developed, based on the separation and detection of alanine, glutamate, and aspartate using capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection. The three amino acids were separated in 5 mM phosphate of pH 2.1 as background electrolyte, and detected on a 500 microm platinum disk electrode at 1.2V (versus Ag/AgCl) in the presence of 10 mM tris(2,2'-bipyridyl)ruthenium(II) dissolved in 80 mM phosphate of pH 10.5. A mass detection limit of 37.3 fmol (or 81.5 fmol) for glutamate, corresponding to the product in the enzyme reaction catalyzed by 1.24 x 10(-9)U AST (or 2.72 x 10(-9)U ALT) in a 30 min reaction period, was achieved. This assay was applied to investigate the cytotoxicity effect of ethanol on HepG2 cells and differentiating nonalcoholic steatohepatitis (NASH) from alcoholic liver disease, indicating that the technique is promising for the application in the cell biological and clinical fields.

Full Text

Duke Authors

Cited Authors

  • Li, T; Yuan, J; Yin, J; Zhang, Z; Wang, E

Published Date

  • November 2006

Published In

Volume / Issue

  • 1134 / 1-2

Start / End Page

  • 311 - 316

PubMed ID

  • 17045597

Pubmed Central ID

  • 17045597

Electronic International Standard Serial Number (EISSN)

  • 1873-3778

International Standard Serial Number (ISSN)

  • 0021-9673

Digital Object Identifier (DOI)

  • 10.1016/j.chroma.2006.08.088

Language

  • eng