Determination of barbituric acid and 2-thiobarbituric acid with end-column electrochemical detection by capillary electrophoresis.
Journal Article
Capillary electrophoresis (CE) with end-column electrochemical detection (EC) of barbituric acid (BA) and 2-thiobarbituric acid (TA) has been described. Under optimum condition, BA and TA were separated satisfactorily, and a response of high sensitivity and stability was obtained at a detection potential of 1.25 V versus Ag/AgCl. Optimized end-column detection provides detection limit as low as 0.5 and 0.1 muM for BA and TA, respectively. The calibration graph was linear over three orders of magnitude. The relative standard deviations (n=10) of peak currents and migration times obtained for both BA and TA were 3.4, 3.7, and 1.7, 1.2%, respectively. The proposed method has been applied to analyze water sample with satisfactory results.
Full Text
Duke Authors
Cited Authors
- You, T; Yang, X; Wang, E
Published Date
- May 5, 2000
Published In
Volume / Issue
- 51 / 6
Start / End Page
- 1213 - 1218
PubMed ID
- 18967953
Pubmed Central ID
- 18967953
Electronic International Standard Serial Number (EISSN)
- 1873-3573
Digital Object Identifier (DOI)
- 10.1016/s0039-9140(00)00312-x
Language
- eng
Conference Location
- Netherlands