Determination of barbituric acid and 2-thiobarbituric acid with end-column electrochemical detection by capillary electrophoresis.

Journal Article (Journal Article)

Capillary electrophoresis (CE) with end-column electrochemical detection (EC) of barbituric acid (BA) and 2-thiobarbituric acid (TA) has been described. Under optimum condition, BA and TA were separated satisfactorily, and a response of high sensitivity and stability was obtained at a detection potential of 1.25 V versus Ag/AgCl. Optimized end-column detection provides detection limit as low as 0.5 and 0.1 muM for BA and TA, respectively. The calibration graph was linear over three orders of magnitude. The relative standard deviations (n=10) of peak currents and migration times obtained for both BA and TA were 3.4, 3.7, and 1.7, 1.2%, respectively. The proposed method has been applied to analyze water sample with satisfactory results.

Full Text

Duke Authors

Cited Authors

  • You, T; Yang, X; Wang, E

Published Date

  • May 5, 2000

Published In

Volume / Issue

  • 51 / 6

Start / End Page

  • 1213 - 1218

PubMed ID

  • 18967953

Electronic International Standard Serial Number (EISSN)

  • 1873-3573

Digital Object Identifier (DOI)

  • 10.1016/s0039-9140(00)00312-x

Language

  • eng

Conference Location

  • Netherlands