Lipid membrane immobilized horseradish peroxidase biosensor for amperometric determination of hydrogen peroxide.

Journal Article (Journal Article)

Stable films of didodecyldimethylammonium bromide (DDAB, a synthetic lipid) and horseradish peroxidase (HRP) were made by casting the mixture of the aqueous vesicle of DDAB and HRP onto the glassy carbon (GC) electrode. The direct electron transfer between electrode and HRP immobilized in lipid film has been demonstrated. The lipid films were used to supply a biological environment resembling biomembrane on the surface of the electrode. A pair of redox peaks attributed to the direct redox reaction of HRP were observed in the phosphate buffer solution (pH 5.5). The cathodic peak current increased dramatically while anodic peak decreased by addition of small amount H(2)O(2). The pH effect on amperometric response to H(2)O(2) was studied. The biosensor also exhibited fast response (5 s), good stability and reproducibility.

Full Text

Duke Authors

Cited Authors

  • Tang, J; Wang, B; Wu, Z; Han, X; Dong, S; Wang, E

Published Date

  • July 2003

Published In

Volume / Issue

  • 18 / 7

Start / End Page

  • 867 - 872

PubMed ID

  • 12713909

International Standard Serial Number (ISSN)

  • 0956-5663

Digital Object Identifier (DOI)

  • 10.1016/s0956-5663(02)00148-3


  • eng

Conference Location

  • England