Comparison of the genomes of the wild-type French viscerotropic strain of yellow fever virus with its vaccine derivative French neurotropic vaccine.

Published

Journal Article

The French neurotropic vaccine, or FNV, was used extensively in Africa to control yellow fever (YF). Although efficacious, the vaccine caused an unacceptable rate of post-vaccinal complications in children and was subsequently replaced by the 17D vaccine. Here we report that the genomes of the wild-type YF virus French viscerotropic virus and its attenuated vaccine derivative, FNV virus from the Institut Pasteur, Paris, (FNV-IP) differ by 77 nucleotides encoding 35 amino acid substitutions. Comparison of FNV-IP and three other isolates of FNV with other YF vaccine strains (17D-204 and 17DD derived from wild-type strain Asibi) revealed that during the two attenuation processes two common nucleotide changes arose that encode two amino acid substitutions: one is in the membrane protein at amino acid 35 (M-35), the other in non-structural (NS) protein 4B at NS4B-95. These common substitutions may be important in the process of attenuation of viscerotropic disease for humans and monkeys, and/or may be involved in loss of mosquito competence of the vaccine viruses.

Full Text

Duke Authors

Cited Authors

  • Wang, E; Ryman, KD; Jennings, AD; Wood, DJ; Taffs, F; Minor, PD; Sanders, PG; Barrett, AD

Published Date

  • November 1995

Published In

Volume / Issue

  • 76 ( Pt 11) /

Start / End Page

  • 2749 - 2755

PubMed ID

  • 7595382

Pubmed Central ID

  • 7595382

Electronic International Standard Serial Number (EISSN)

  • 1465-2099

International Standard Serial Number (ISSN)

  • 0022-1317

Digital Object Identifier (DOI)

  • 10.1099/0022-1317-76-11-2749

Language

  • eng