Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.

Published

Journal Article

Simian virus 40 T antigen has been shown previously to bind specifically with high affinity to sites within the regulatory region of double-stranded simian virus 40 DNA. Using competition filter binding and the DNA-binding immunoassay, we show that T antigen did not bind specifically to either early or late single-stranded DNA containing these binding sites. Moreover, T antigen did not bind these sequences present in single-stranded RNA, RNA/RNA duplexes, or RNA/DNA hybrids. T antigen did, however, bind as efficiently to single-stranded DNA-cellulose as to double-stranded DNA-cellulose. This binding was nonspecific because it was independent of the presence of T-antigen-binding sites. The implications of these observations are discussed.

Full Text

Duke Authors

Cited Authors

  • Auborn, KJ; Markowitz, RB; Wang, E; Yu, YT; Prives, C

Published Date

  • June 1988

Published In

Volume / Issue

  • 62 / 6

Start / End Page

  • 2204 - 2208

PubMed ID

  • 3367427

Pubmed Central ID

  • 3367427

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States