Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.
Journal Article (Journal Article)
Simian virus 40 T antigen has been shown previously to bind specifically with high affinity to sites within the regulatory region of double-stranded simian virus 40 DNA. Using competition filter binding and the DNA-binding immunoassay, we show that T antigen did not bind specifically to either early or late single-stranded DNA containing these binding sites. Moreover, T antigen did not bind these sequences present in single-stranded RNA, RNA/RNA duplexes, or RNA/DNA hybrids. T antigen did, however, bind as efficiently to single-stranded DNA-cellulose as to double-stranded DNA-cellulose. This binding was nonspecific because it was independent of the presence of T-antigen-binding sites. The implications of these observations are discussed.
Full Text
Duke Authors
Cited Authors
- Auborn, KJ; Markowitz, RB; Wang, E; Yu, YT; Prives, C
Published Date
- June 1988
Published In
Volume / Issue
- 62 / 6
Start / End Page
- 2204 - 2208
PubMed ID
- 3367427
Pubmed Central ID
- PMC253332
International Standard Serial Number (ISSN)
- 0022-538X
Digital Object Identifier (DOI)
- 10.1128/JVI.62.6.2204-2208.1988
Language
- eng
Conference Location
- United States