Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells.

Published

Journal Article

Rab10, a protein originally isolated from Madin-Darby Canine Kidney (MDCK) epithelial cells, belongs to a family of Rab proteins that includes Rab8 and Rab13. Although both Rab8 and Rab13 have been found to mediate polarized membrane transport, the function of Rab10 in mammalian cells has not yet been established. We have used quantitative confocal microscopy of polarized MDCK cells expressing GFP chimeras of wild-type and mutant forms of Rab10 to analyze the function of Rab10 in polarized cells. These studies demonstrate that Rab10 is specifically associated with the common endosomes of MDCK cells, accessible to endocytic probes internalized from either the apical or basolateral plasma membrane domains. Expression of mutant Rab10 defective for either GTP hydrolysis or GTP binding increased recycling from early compartments on the basolateral endocytic pathway without affecting recycling from later compartments or the apical recycling pathway. These results suggest that Rab10 mediates transport from basolateral sorting endosomes to common endosomes.

Full Text

Duke Authors

Cited Authors

  • Babbey, CM; Ahktar, N; Wang, E; Chen, CC-H; Grant, BD; Dunn, KW

Published Date

  • July 2006

Published In

Volume / Issue

  • 17 / 7

Start / End Page

  • 3156 - 3175

PubMed ID

  • 16641372

Pubmed Central ID

  • 16641372

Electronic International Standard Serial Number (EISSN)

  • 1939-4586

International Standard Serial Number (ISSN)

  • 1059-1524

Digital Object Identifier (DOI)

  • 10.1091/mbc.E05-08-0799

Language

  • eng