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Phenotypic variation in the response to the human immunodeficiency virus among derivatives of the CEM T and WIL-2 B cell lines.

Publication ,  Journal Article
Chaffee, S; Leeds, JM; Matthews, TJ; Weinhold, KJ; Skinner, M; Bolognesi, DP; Hershfield, MS
Published in: J Exp Med
August 1, 1988

Derivatives of the CEM T and WIL-2 B cell lines showed striking diversity in their responses to the HTLV-IIIB strain of the human immunodeficiency virus (HIV). Several stable phenotypic patterns could be defined, based on whether cells were permissive (P+, P-) for virus production, were sensitive or insensitive to cytopathic effects after infection by free virus (C+, C-), and whether they underwent fusion on contact with virus-infected cells (F+, F-). Although expression of CD4 was essential for infection by HTLV-IIIB, very low levels were sufficient for productive infection of WIL-2 derivatives. Conversely, some CEM T cell lines that expressed ample CD4, and which were able to bind virus gp120 and undergo fusion, did not support productive infection by free virus. One nonpermissive, CD4+ derivative of CEM could bind gp120 but failed to undergo fusion, suggesting an alteration in some membrane protein other than CD4 that is essential for virus entry and HIV-induced cell fusion. The AA2 derivative of the WIL-2 cell line is also described, which is remarkably permissive for HIV replication and exquisitely sensitive to virus cytopathic effect. The panel of related cell lines with different host-virus phenotypes could be useful for more precisely defining steps in the infectious cycle of HIV, and for identifying host cell genes and gene products that determine the outcome of HIV infection.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

August 1, 1988

Volume

168

Issue

2

Start / End Page

605 / 621

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Receptors, Virus
  • Phenotype
  • Mutation
  • Immunology
  • Humans
  • HIV
  • Cell Line
  • B-Lymphocytes
  • Antigens, Surface
 

Citation

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ICMJE
MLA
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Chaffee, S., Leeds, J. M., Matthews, T. J., Weinhold, K. J., Skinner, M., Bolognesi, D. P., & Hershfield, M. S. (1988). Phenotypic variation in the response to the human immunodeficiency virus among derivatives of the CEM T and WIL-2 B cell lines. J Exp Med, 168(2), 605–621. https://doi.org/10.1084/jem.168.2.605
Chaffee, S., J. M. Leeds, T. J. Matthews, K. J. Weinhold, M. Skinner, D. P. Bolognesi, and M. S. Hershfield. “Phenotypic variation in the response to the human immunodeficiency virus among derivatives of the CEM T and WIL-2 B cell lines.J Exp Med 168, no. 2 (August 1, 1988): 605–21. https://doi.org/10.1084/jem.168.2.605.
Chaffee S, Leeds JM, Matthews TJ, Weinhold KJ, Skinner M, Bolognesi DP, et al. Phenotypic variation in the response to the human immunodeficiency virus among derivatives of the CEM T and WIL-2 B cell lines. J Exp Med. 1988 Aug 1;168(2):605–21.
Chaffee, S., et al. “Phenotypic variation in the response to the human immunodeficiency virus among derivatives of the CEM T and WIL-2 B cell lines.J Exp Med, vol. 168, no. 2, Aug. 1988, pp. 605–21. Pubmed, doi:10.1084/jem.168.2.605.
Chaffee S, Leeds JM, Matthews TJ, Weinhold KJ, Skinner M, Bolognesi DP, Hershfield MS. Phenotypic variation in the response to the human immunodeficiency virus among derivatives of the CEM T and WIL-2 B cell lines. J Exp Med. 1988 Aug 1;168(2):605–621.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

August 1, 1988

Volume

168

Issue

2

Start / End Page

605 / 621

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Receptors, Virus
  • Phenotype
  • Mutation
  • Immunology
  • Humans
  • HIV
  • Cell Line
  • B-Lymphocytes
  • Antigens, Surface