The coenrichment of stem cells, prothymocytes, and stromal elements with ecotropic retrovirus-producing cells from the bone marrow of leukemia-prone AKR mice.

Journal Article (Journal Article)

Ecotropic virus-producing cells in the bone marrow of the leukemia-prone AKR strain of mice were significantly enriched by fractionation on discontinuous density gradients of Percoll and were found in a low-density population of cells comprised predominantly of medium to large blast cells. The high ecotropic virus-producing low-density bone marrow cell population was also found to be significantly enriched in pluripotent stem cells, prothymocytes, and stromal elements. During the period of time defined by a window for successful leukemosuppressive immunotherapy of AKR mice, virus-producing cells were exclusively detected in this fraction of bone marrow cells, implicating the functional classes of cells coenriched in this fraction as both potential targets of anti-viral immunotherapy and responsible for the seeding of the spleen and thymus with infectious ecotropic virus.

Full Text

Duke Authors

Cited Authors

  • Buckheit, RW; Kurtzberg, J; Bolognesi, DP; Weinhold, KJ

Published Date

  • February 1, 1988

Published In

Volume / Issue

  • 162 / 2

Start / End Page

  • 354 - 361

PubMed ID

  • 2829423

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/0042-6822(88)90475-8


  • eng

Conference Location

  • United States