Skip to main content
Journal cover image

Epidermal growth factor receptor expression and gene copy number in fibrolamellar hepatocellular carcinoma.

Publication ,  Journal Article
Buckley, AF; Burgart, LJ; Kakar, S
Published in: Hum Pathol
April 2006

Increased expression of epidermal growth factor receptor (EGFR), a transmembrane tyrosine kinase, is associated with tumor progression in many carcinomas. Epidermal growth factor receptor inhibitors have shown promise in treating some of these tumors. Fibrolamellar hepatocellular carcinoma (FL-HCC) is an aggressive neoplasm that occurs in young patients with no history of cirrhosis. This study examines the expression and gene copy number of EGFR in FL-HCC. Formalin-fixed, paraffin-embedded FL-HCC (n = 13) sections were stained with a monoclonal antibody against EGFR. Fluorescence in situ hybridization analysis was performed using probes against EGFR gene and centromeric region of chromosome 7 (CEP 7). Epidermal growth factor receptor and CEP 7 signals were counted in 50 tumor nuclei per case as well as 300 normal hepatocyte nuclei. The EGFR to CEP 7 signal ratio was calculated for each case. Most (92%, 12/13) of FL-HCC showed strong and diffuse staining with anti-EGFR antibody. Fluorescence in situ hybridization was informative in 11 cases, 10 of which showed extra EGFR gene copy numbers (mean, 3.69; range, 3.13-5.0). Epidermal growth factor receptor was overexpressed in all these cases. The mean number of EGFR signals per cell in FL-HCC was double that of normal hepatocytes (3.69 versus 1.80); the mean EGFR/CEP 7 ratio in tumor cells was 1.05. In conclusion, EGFR is strongly overexpressed on the cell membrane in nearly all cases of FL-HCC. Similar gains of chromosome 7 are observed, indicating that the extra EGFR gene copies are due to polysomy rather than gene amplification. The strong expression of EGFR in FL-HCC tumors suggests that they may respond to treatment with EGFR antagonists.

Duke Scholars

Published In

Hum Pathol

DOI

ISSN

0046-8177

Publication Date

April 2006

Volume

37

Issue

4

Start / End Page

410 / 414

Location

United States

Related Subject Headings

  • Pathology
  • Middle Aged
  • Male
  • Liver Neoplasms
  • In Situ Hybridization, Fluorescence
  • Immunoenzyme Techniques
  • Humans
  • Hepatocytes
  • Gene Expression Regulation, Neoplastic
  • Gene Dosage
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Buckley, A. F., Burgart, L. J., & Kakar, S. (2006). Epidermal growth factor receptor expression and gene copy number in fibrolamellar hepatocellular carcinoma. Hum Pathol, 37(4), 410–414. https://doi.org/10.1016/j.humpath.2005.12.009
Buckley, Anne F., Lawrence J. Burgart, and Sanjay Kakar. “Epidermal growth factor receptor expression and gene copy number in fibrolamellar hepatocellular carcinoma.Hum Pathol 37, no. 4 (April 2006): 410–14. https://doi.org/10.1016/j.humpath.2005.12.009.
Buckley, Anne F., et al. “Epidermal growth factor receptor expression and gene copy number in fibrolamellar hepatocellular carcinoma.Hum Pathol, vol. 37, no. 4, Apr. 2006, pp. 410–14. Pubmed, doi:10.1016/j.humpath.2005.12.009.
Journal cover image

Published In

Hum Pathol

DOI

ISSN

0046-8177

Publication Date

April 2006

Volume

37

Issue

4

Start / End Page

410 / 414

Location

United States

Related Subject Headings

  • Pathology
  • Middle Aged
  • Male
  • Liver Neoplasms
  • In Situ Hybridization, Fluorescence
  • Immunoenzyme Techniques
  • Humans
  • Hepatocytes
  • Gene Expression Regulation, Neoplastic
  • Gene Dosage