Skip to main content
Journal cover image

NF-kappaB inhibition by an adenovirus expressed aptamer sensitizes TNFalpha-induced apoptosis.

Publication ,  Journal Article
Mi, J; Zhang, X; Liu, Y; Reddy, SK; Rabbani, ZN; Sullenger, BA; Clary, BM
Published in: Biochem Biophys Res Commun
August 3, 2007

Prolonged activation of NF-kappaB is involved in the pathogenesis of chronic inflammatory diseases and associated cancers. NF-kappaB activation is considered to be a main mechanism opposing TNFalpha-induced apoptosis. We investigated whether inhibition of NF-kappaB could sensitize tumor and endothelial cells to TNFalpha-induced apoptosis. As such, we developed a novel H1 RNA polymerase III promoter driven adenoviral vector to express an RNA aptamer, Ad-A-p50, which selectively inhibits NF-kappaB activation in the nucleus. This event sensitizes human lung adenocarcinoma cells (A549) and human endothelial cells (HUVEC) to TNFalpha-induced apoptosis through the multiple pathways regulated by NF-kappaB, including Bcl-XL, HIF-1alpha, and VEGF. Our findings also suggest a new mechanism of HIF-1alpha regulation by NF-kappaB in the normoxic environment. RNA aptamer inhibition of NF-kappaB offers exciting opportunities for sensitizing inflammatory and tumor cells to TNFalpha-induced apoptosis.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 3, 2007

Volume

359

Issue

3

Start / End Page

475 / 480

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Neoplasms
  • NF-kappa B
  • Molecular Sequence Data
  • Humans
  • Endothelial Cells
  • Down-Regulation
  • Cells, Cultured
  • Biochemistry & Molecular Biology
  • Base Sequence
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mi, J., Zhang, X., Liu, Y., Reddy, S. K., Rabbani, Z. N., Sullenger, B. A., & Clary, B. M. (2007). NF-kappaB inhibition by an adenovirus expressed aptamer sensitizes TNFalpha-induced apoptosis. Biochem Biophys Res Commun, 359(3), 475–480. https://doi.org/10.1016/j.bbrc.2007.05.125
Mi, Jing, Xiuwu Zhang, Yingmiao Liu, Srinevas K. Reddy, Zahid N. Rabbani, Bruce A. Sullenger, and Bryan M. Clary. “NF-kappaB inhibition by an adenovirus expressed aptamer sensitizes TNFalpha-induced apoptosis.Biochem Biophys Res Commun 359, no. 3 (August 3, 2007): 475–80. https://doi.org/10.1016/j.bbrc.2007.05.125.
Mi J, Zhang X, Liu Y, Reddy SK, Rabbani ZN, Sullenger BA, et al. NF-kappaB inhibition by an adenovirus expressed aptamer sensitizes TNFalpha-induced apoptosis. Biochem Biophys Res Commun. 2007 Aug 3;359(3):475–80.
Mi, Jing, et al. “NF-kappaB inhibition by an adenovirus expressed aptamer sensitizes TNFalpha-induced apoptosis.Biochem Biophys Res Commun, vol. 359, no. 3, Aug. 2007, pp. 475–80. Pubmed, doi:10.1016/j.bbrc.2007.05.125.
Mi J, Zhang X, Liu Y, Reddy SK, Rabbani ZN, Sullenger BA, Clary BM. NF-kappaB inhibition by an adenovirus expressed aptamer sensitizes TNFalpha-induced apoptosis. Biochem Biophys Res Commun. 2007 Aug 3;359(3):475–480.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 3, 2007

Volume

359

Issue

3

Start / End Page

475 / 480

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Neoplasms
  • NF-kappa B
  • Molecular Sequence Data
  • Humans
  • Endothelial Cells
  • Down-Regulation
  • Cells, Cultured
  • Biochemistry & Molecular Biology
  • Base Sequence