Identification of OTX2 as a medulloblastoma oncogene whose product can be targeted by all-trans retinoic acid.

Published

Journal Article

Through digital karyotyping of permanent medulloblastoma cell lines, we found that the homeobox gene OTX2 was amplified more than 10-fold in three cell lines. Gene expression analyses showed that OTX2 transcripts were present at high levels in 14 of 15 (93%) medulloblastomas with anaplastic histopathologic features. Knockdown of OTX2 expression by siRNAs inhibited medulloblastoma cell growth in vitro, whereas pharmacologic doses of all-trans retinoic acid repressed OTX2 expression and induced apoptosis only in medulloblastoma cell lines that expressed OTX2. These observations suggest that OTX2 is essential for the pathogenesis of anaplastic medulloblastomas and that these tumors may be amenable to therapy with all-trans-retinoic acid.

Full Text

Duke Authors

Cited Authors

  • Di, C; Liao, S; Adamson, DC; Parrett, TJ; Broderick, DK; Shi, Q; Lengauer, C; Cummins, JM; Velculescu, VE; Fults, DW; McLendon, RE; Bigner, DD; Yan, H

Published Date

  • February 1, 2005

Published In

Volume / Issue

  • 65 / 3

Start / End Page

  • 919 - 924

PubMed ID

  • 15705891

Pubmed Central ID

  • 15705891

International Standard Serial Number (ISSN)

  • 0008-5472

Language

  • eng

Conference Location

  • United States