Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells.
Journal Article (Journal Article)
Phosphorodiamidate morpholino oligomers (PMO), which have a neutral chemistry, are extensively being used as tools for selective inhibition of gene expression in cell culture models and are currently in human clinical trials. PMO oligomers possess a unique structure, in which the deoxyribose moiety of DNA is replaced with a six-membered morpholine ring and the charged phosphodiester internucleoside linkages are replaced with neutral phosphorodiamidate linkages. PMO internalization in uptake-permissive cells has been observed to be specific, saturable, and energy-dependent, suggesting a receptor-mediated uptake mechanism. Understanding PMO transport should facilitate the design of more effective synthetic antisense oligomers as therapeutic agents.
Full Text
Duke Authors
Cited Authors
- Devi, GR
Published Date
- 2009
Published In
Volume / Issue
- 542 /
Start / End Page
- 351 - 361
PubMed ID
- 19565912
International Standard Serial Number (ISSN)
- 1064-3745
Digital Object Identifier (DOI)
- 10.1007/978-1-59745-561-9_19
Language
- eng
Conference Location
- United States