RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus.


Journal Article

The Dlk1-Gtl2 imprinted domain, encompassing the callipyge (CLPG) locus in sheep, has recently been shown to harbor a large number of maternally expressed miRNA genes [1, 2]. Two of these (mir127 and mir136) are processed from a transcript (antiPeg11) that is antisense to Rtl1/Peg11, a paternally expressed intronless gene with homology to the gag and pol polyproteins of Sushi-like retroelements [3]. We herein demonstrate that several additional miRNAs are processed from antiPeg11 and that these regulate Rtl1/Peg11 in trans by guiding RISC-mediated cleavage of its mRNA. This is the first demonstration of miRNA-mediated RNAi involving imprinted genes in mammals.

Full Text

Cited Authors

  • Davis, E; Caiment, F; Tordoir, X; Cavaillé, J; Ferguson-Smith, A; Cockett, N; Georges, M; Charlier, C

Published Date

  • April 2005

Published In

Volume / Issue

  • 15 / 8

Start / End Page

  • 743 - 749

PubMed ID

  • 15854907

Pubmed Central ID

  • 15854907

Electronic International Standard Serial Number (EISSN)

  • 1879-0445

International Standard Serial Number (ISSN)

  • 0960-9822

Digital Object Identifier (DOI)

  • 10.1016/j.cub.2005.02.060


  • eng