The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints.

Journal Article (Journal Article)

Although polysaccharide intercellular adhesin (PIA) is thought to be crucial in the pathogenesis of prosthetic device infections caused by Staphylococcus epidermidis, its role in prosthetic device infections caused by Staphylococcus aureus is unknown. To assess the clinical impact of PIA production, isolates from 15 prospectively identified cases of S. aureus bacteremia in patients with prosthetic joints (8 infected, 7 uninfected) were characterized for biofilm production, hemagglutination, and the presence of a 419-bp amplification product within icaA. Although icaA was present in all 15 isolates, none of the isolates produced hemagglutination and only one isolate (from a patient with an uninfected prosthetic device) weakly produced biofilm in vitro. These results support the observation that the ica locus is conserved between S. epidermidis and S. aureus and that PIA may be expressed only under in vivo conditions. Future investigations should include animal models to approximate the complex milieu surrounding implanted prosthetic medical devices.

Full Text

Duke Authors

Cited Authors

  • Fowler, VG; Fey, PD; Reller, LB; Chamis, AL; Corey, GR; Rupp, ME

Published Date

  • April 2001

Published In

Volume / Issue

  • 189 / 3

Start / End Page

  • 127 - 131

PubMed ID

  • 11388609

International Standard Serial Number (ISSN)

  • 0300-8584

Digital Object Identifier (DOI)

  • 10.1007/s430-001-8018-5


  • eng

Conference Location

  • Germany