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Immunotherapy using allogeneic squamous cell tumor-dendritic cell fusion hybrids.

Publication ,  Journal Article
Lee, WT; Tan, C; Koski, G; Shu, S; Cohen, P
Published in: Head Neck
September 2010

BACKGROUND: Tumor-associated antigens (TAAs) are known to be immunotherapy targets; thus tumor-sharing TAA may be used as a fusion hybrid partner to confer protection against subsequent tumor challenge. METHODS: The squamous cell carcinomas (SCCs), SCCVII and B4B8, were used in C3H/HEN mice: SCCVII (H-2(k)) is syngeneic, B4B8 (H-2(d)) is allogeneic. Experiments using tumor alone included hyperimmunization schedule, subdermal and intranodal routes. Mice were challenged 2 weeks later. Fusion hybrids were created from both SCC tumor cell lines and syngeneic dendritic cells (DCs). These were delivered intranodally for immunization, and mice were challenged with tumor 2 weeks later. RESULTS: Only syngeneic tumor given subdermally was able to protect after tumor challenge 2 weeks later. Hyperimmunization schedule did not alter these findings. However, fusion hybrid immunization from both allogeneic and syngeneic SCCs conferred protection after tumor challenge. CONCLUSIONS: Allogeneic tumor-DC fusion hybrids targeting TAA can protect against subsequent tumor challenge.

Duke Scholars

Published In

Head Neck

DOI

EISSN

1097-0347

Publication Date

September 2010

Volume

32

Issue

9

Start / End Page

1209 / 1216

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Sensitivity and Specificity
  • Random Allocation
  • Otorhinolaryngology
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
  • Mice
  • Interleukin-18
  • Immunotherapy
  • Hybrid Cells
 

Citation

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Lee, W. T., Tan, C., Koski, G., Shu, S., & Cohen, P. (2010). Immunotherapy using allogeneic squamous cell tumor-dendritic cell fusion hybrids. Head Neck, 32(9), 1209–1216. https://doi.org/10.1002/hed.21323
Lee, Walter T., Chunrui Tan, Gary Koski, Suyu Shu, and Peter Cohen. “Immunotherapy using allogeneic squamous cell tumor-dendritic cell fusion hybrids.Head Neck 32, no. 9 (September 2010): 1209–16. https://doi.org/10.1002/hed.21323.
Lee WT, Tan C, Koski G, Shu S, Cohen P. Immunotherapy using allogeneic squamous cell tumor-dendritic cell fusion hybrids. Head Neck. 2010 Sep;32(9):1209–16.
Lee, Walter T., et al. “Immunotherapy using allogeneic squamous cell tumor-dendritic cell fusion hybrids.Head Neck, vol. 32, no. 9, Sept. 2010, pp. 1209–16. Pubmed, doi:10.1002/hed.21323.
Lee WT, Tan C, Koski G, Shu S, Cohen P. Immunotherapy using allogeneic squamous cell tumor-dendritic cell fusion hybrids. Head Neck. 2010 Sep;32(9):1209–1216.
Journal cover image

Published In

Head Neck

DOI

EISSN

1097-0347

Publication Date

September 2010

Volume

32

Issue

9

Start / End Page

1209 / 1216

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Sensitivity and Specificity
  • Random Allocation
  • Otorhinolaryngology
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
  • Mice
  • Interleukin-18
  • Immunotherapy
  • Hybrid Cells