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Thermodynamics of DNA hybridization on gold nanoparticles.

Publication ,  Journal Article
Xu, J; Craig, SL
Published in: Journal of the American Chemical Society
September 2005

Dynamic light scattering is used as a sensitive probe of hybridization on DNA-functionalized colloidal gold nanoparticles. When a target DNA strand possesses an 8 base "dangling end", duplex formation on the surface of the nanoparticles leads to an increase in hydrodynamic radius. Duplex melting is manifested in a drop in hydrodynamic radius with increasing temperature, and the concentration dependence of the melting temperature provides a measure of the thermodynamics of binding. The hybridization thermodynamics are found to be significantly lower at higher hybridization densities than those previously reported for initial hybridization events. The pronounced deviation from Langmuir adsorption behavior is greater for longer duplexes, and it is, therefore, consistent with electrostatic repulsion between densely packed oligonucleotides. The results have implications for sensing and DNA-directed nanoparticle assembly.

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Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

September 2005

Volume

127

Issue

38

Start / End Page

13227 / 13231

Related Subject Headings

  • Thermodynamics
  • Temperature
  • Surface Properties
  • Nucleic Acid Hybridization
  • Nanostructures
  • Gold
  • General Chemistry
  • DNA
  • 40 Engineering
  • 34 Chemical sciences
 

Citation

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Xu, J., & Craig, S. L. (2005). Thermodynamics of DNA hybridization on gold nanoparticles. Journal of the American Chemical Society, 127(38), 13227–13231. https://doi.org/10.1021/ja052352h
Xu, Jun, and Stephen L. Craig. “Thermodynamics of DNA hybridization on gold nanoparticles.Journal of the American Chemical Society 127, no. 38 (September 2005): 13227–31. https://doi.org/10.1021/ja052352h.
Xu J, Craig SL. Thermodynamics of DNA hybridization on gold nanoparticles. Journal of the American Chemical Society. 2005 Sep;127(38):13227–31.
Xu, Jun, and Stephen L. Craig. “Thermodynamics of DNA hybridization on gold nanoparticles.Journal of the American Chemical Society, vol. 127, no. 38, Sept. 2005, pp. 13227–31. Epmc, doi:10.1021/ja052352h.
Xu J, Craig SL. Thermodynamics of DNA hybridization on gold nanoparticles. Journal of the American Chemical Society. 2005 Sep;127(38):13227–13231.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

September 2005

Volume

127

Issue

38

Start / End Page

13227 / 13231

Related Subject Headings

  • Thermodynamics
  • Temperature
  • Surface Properties
  • Nucleic Acid Hybridization
  • Nanostructures
  • Gold
  • General Chemistry
  • DNA
  • 40 Engineering
  • 34 Chemical sciences