Regulation of nuclear Prointerleukin-16 and p27(Kip1) in primary human T lymphocytes.

Journal Article

Prointerleukin-16 (Pro-IL-16) is an abundant, PDZ domain-containing protein expressed in the nucleus and cytoplasm of resting human T lymphocytes. We have previously shown that ectopic expression of Pro-IL-16 in Pro-IL-16-negative human Jurkat cells represses transcription of the F-box protein, Skp2, resulting in accumulation of the cyclin-dependent kinase inhibitor, p27(Kip1), and G0/G1 cell cycle arrest. The current studies demonstrate the kinetics of Pro-IL-16 and p27(Kip1) expression in activated normal human T lymphocytes. We correlate nuclear Pro-IL-16 loss with decreased p27(Kip1) expression, increased cell cycle progression, and proliferation. Conversely, we show that constitutive expression of Pro-IL-16 by retroviral infection of activated human T lymphocytes induces G0/G1 cell cycle arrest, inhibits proliferation, and is associated with increased levels of p27(Kip1). These findings implicate nuclear Pro-IL-16 as a cell cycle regulatory protein for human T lymphocytes.

Full Text

Duke Authors

Cited Authors

  • Wilson, KC; Cattel, DJ; Wan, Z; Rahangdale, S; Ren, F; Kornfeld, H; Sullivan, BA; Cruikshank, WW; Center, DM

Published Date

  • September 2005

Published In

Volume / Issue

  • 237 / 1

Start / End Page

  • 17 - 27

PubMed ID

  • 16289056

International Standard Serial Number (ISSN)

  • 0008-8749

Digital Object Identifier (DOI)

  • 10.1016/j.cellimm.2005.09.003

Language

  • eng

Conference Location

  • Netherlands