Clarification of subtle reciprocal rearrangements using fluorescence in situ hybridization.

Journal Article (Journal Article)

Fluorescence in situ hybridization (FISH) using chromosome-specific DNA libraries as painting probes was applied in the analysis of six subtle, balanced chromosome rearrangements. Both fresh and older slides, some of which had been previously G-banded, were used to determine if FISH could identify unambiguously very small amounts of translocated material. Our results indicate that this procedure can clearly and precisely distinguish the specific components of extremely subtle translocations, in different cell types, such as leukocytes, aminocytes, and chorionic villus, and irregardless of preparation age. This ability makes FISH a valuable tool in clinical cytogenetics for the confirmation of preliminary G-banded karyotypes.

Full Text

Duke Authors

Cited Authors

  • Sullivan, BA; Leana-Cox, J; Schwartz, S

Published Date

  • August 15, 1993

Published In

Volume / Issue

  • 47 / 2

Start / End Page

  • 223 - 230

PubMed ID

  • 8213910

International Standard Serial Number (ISSN)

  • 0148-7299

Digital Object Identifier (DOI)

  • 10.1002/ajmg.1320470217


  • eng

Conference Location

  • United States