Conserved organization of centromeric chromatin in flies and humans.

Journal Article (Journal Article)

Recent studies have highlighted the importance of centromere-specific histone H3-like (CENP-A) proteins in centromere function. We show that Drosophila CID and human CENP-A appear at metaphase as a three-dimensional structure that lacks histone H3. However, blocks of CID/CENP-A and H3 nucleosomes are linearly interspersed on extended chromatin fibers, and CID is close to H3 nucleosomes in polynucleosomal preparations. When CID is depleted by RNAi, it is replaced by H3, demonstrating flexibility of centromeric chromatin organization. Finally, contrary to models proposing that H3 and CID/CENP-A nucleosomes are replicated at different times in S phase, we show that interspersed H3 and CID/CENP-A chromatin are replicated concurrently during S phase in humans and flies. We propose that the unique structural arrangement of CID/CENP-A and H3 nucleosomes presents centromeric chromatin to the poleward face of the condensing mitotic chromosome.

Full Text

Duke Authors

Cited Authors

  • Blower, MD; Sullivan, BA; Karpen, GH

Published Date

  • March 2002

Published In

Volume / Issue

  • 2 / 3

Start / End Page

  • 319 - 330

PubMed ID

  • 11879637

Pubmed Central ID

  • PMC3192492

International Standard Serial Number (ISSN)

  • 1534-5807

Digital Object Identifier (DOI)

  • 10.1016/s1534-5807(02)00135-1


  • eng

Conference Location

  • United States