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Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing.

Publication ,  Journal Article
Kim, J-H; Ebersole, T; Kouprina, N; Noskov, VN; Ohzeki, J-I; Masumoto, H; Mravinac, B; Sullivan, BA; Pavlicek, A; Dovat, S; Pack, SD ...
Published in: Genome Res
April 2009

The role of repetitive DNA sequences in pericentromeric regions with respect to kinetochore/heterochromatin structure and function is poorly understood. Here, we use a mouse erythroleukemia cell (MEL) system for studying how repetitive DNA assumes or is assembled into different chromatin structures. We show that human gamma-satellite DNA arrays allow a transcriptionally permissive chromatin conformation in an adjacent transgene and efficiently protect it from epigenetic silencing. These arrays contain CTCF and Ikaros binding sites. In MEL cells, this gamma-satellite DNA activity depends on binding of Ikaros proteins involved in differentiation along the hematopoietic pathway. Given our discovery of gamma-satellite DNA in pericentromeric regions of most human chromosomes and a dynamic chromatin state of gamma-satellite arrays in their natural location, we suggest that gamma-satellite DNA represents a unique region of the functional centromere with a possible role in preventing heterochromatin spreading beyond the pericentromeric region.

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Published In

Genome Res

DOI

ISSN

1088-9051

Publication Date

April 2009

Volume

19

Issue

4

Start / End Page

533 / 544

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transgenes
  • Repressor Proteins
  • Repetitive Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Phylogeny
  • Mice
  • Luciferases
  • Leukemia, Erythroblastic, Acute
  • Ikaros Transcription Factor
 

Citation

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Kim, J.-H., Ebersole, T., Kouprina, N., Noskov, V. N., Ohzeki, J.-I., Masumoto, H., … Larionov, V. (2009). Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing. Genome Res, 19(4), 533–544. https://doi.org/10.1101/gr.086496.108
Kim, Jung-Hyun, Thomas Ebersole, Natalay Kouprina, Vladimir N. Noskov, Jun-Ichirou Ohzeki, Hiroshi Masumoto, Brankica Mravinac, et al. “Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing.Genome Res 19, no. 4 (April 2009): 533–44. https://doi.org/10.1101/gr.086496.108.
Kim J-H, Ebersole T, Kouprina N, Noskov VN, Ohzeki J-I, Masumoto H, et al. Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing. Genome Res. 2009 Apr;19(4):533–44.
Kim, Jung-Hyun, et al. “Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing.Genome Res, vol. 19, no. 4, Apr. 2009, pp. 533–44. Pubmed, doi:10.1101/gr.086496.108.
Kim J-H, Ebersole T, Kouprina N, Noskov VN, Ohzeki J-I, Masumoto H, Mravinac B, Sullivan BA, Pavlicek A, Dovat S, Pack SD, Kwon Y-W, Flanagan PT, Loukinov D, Lobanenkov V, Larionov V. Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing. Genome Res. 2009 Apr;19(4):533–544.

Published In

Genome Res

DOI

ISSN

1088-9051

Publication Date

April 2009

Volume

19

Issue

4

Start / End Page

533 / 544

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transgenes
  • Repressor Proteins
  • Repetitive Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Phylogeny
  • Mice
  • Luciferases
  • Leukemia, Erythroblastic, Acute
  • Ikaros Transcription Factor