Activity of a type 1 picornavirus internal ribosomal entry site is determined by sequences within the 3' nontranslated region.

Journal Article (Journal Article)

We have proposed a cancer treatment modality based on poliovirus chimeras replicating under the translational control of an internal ribosomal entry site (IRES) derived from human rhinovirus type 2. Insertion of the heterologous IRES causes a neuron-specific propagation deficit and eliminates neurovirulence inherent in poliovirus without affecting viral growth in cells derived from malignant gliomas. We now report the elucidation of a molecular mechanism responsible for the cell type-specific defect mediated by the rhinovirus IRES. Rhinovirus IRES function in neuronal cell types depends on specific structural elements within the 3' non-translated region of the viral genome. Our observations suggest long-range interactions between the IRES and the 3' terminus that control IRES-mediated gene expression and virus propagation.

Full Text

Duke Authors

Cited Authors

  • Dobrikova, E; Florez, P; Bradrick, S; Gromeier, M

Published Date

  • December 9, 2003

Published In

Volume / Issue

  • 100 / 25

Start / End Page

  • 15125 - 15130

PubMed ID

  • 14645707

Pubmed Central ID

  • PMC299919

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.2436464100


  • eng

Conference Location

  • United States