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Expression of the human poliovirus receptor/CD155 gene is activated by sonic hedgehog.

Publication ,  Journal Article
Solecki, DJ; Gromeier, M; Mueller, S; Bernhardt, G; Wimmer, E
Published in: J Biol Chem
July 12, 2002

The human poliovirus receptor/CD155 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The ectodomain of CD155 mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1. CD155 is a primate-restricted gene that is expressed during development in mesenchymal tissues and ventrally derived structures within the CNS. Its function in adults is as yet unknown, but significantly, CD155 is aberrantly expressed in neuroectodermal tumors. We show that the expression of CD155 mRNA is up-regulated when human Ntera2 cells are treated with purified Sonic hedgehog (Shh) protein. Reporter gene expression driven by the CD155 core promoter is activated by Shh in transient co-transfection assays. Analysis of the CD155 core promoter indicates that an intact GLI binding site is required for Shh activation. In addition, overexpression of Gli1 or Gli3 potently activates reporter gene expression driven by the CD155 core promoter. These data identify the CD155 gene as a transcriptional target of Shh, a finding that has significance for the normal function of CD155 during development and the expression of CD155 in neuroectodermal tumors.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 12, 2002

Volume

277

Issue

28

Start / End Page

25697 / 25702

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Trans-Activators
  • Receptors, Virus
  • Promoter Regions, Genetic
  • Mice, Transgenic
  • Mice, Inbred C3H
  • Mice
  • Membrane Proteins
  • Humans
  • Hedgehog Proteins
 

Citation

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Solecki, D. J., Gromeier, M., Mueller, S., Bernhardt, G., & Wimmer, E. (2002). Expression of the human poliovirus receptor/CD155 gene is activated by sonic hedgehog. J Biol Chem, 277(28), 25697–25702. https://doi.org/10.1074/jbc.M201378200
Solecki, David J., Matthias Gromeier, Steffen Mueller, Günter Bernhardt, and Eckard Wimmer. “Expression of the human poliovirus receptor/CD155 gene is activated by sonic hedgehog.J Biol Chem 277, no. 28 (July 12, 2002): 25697–702. https://doi.org/10.1074/jbc.M201378200.
Solecki DJ, Gromeier M, Mueller S, Bernhardt G, Wimmer E. Expression of the human poliovirus receptor/CD155 gene is activated by sonic hedgehog. J Biol Chem. 2002 Jul 12;277(28):25697–702.
Solecki, David J., et al. “Expression of the human poliovirus receptor/CD155 gene is activated by sonic hedgehog.J Biol Chem, vol. 277, no. 28, July 2002, pp. 25697–702. Pubmed, doi:10.1074/jbc.M201378200.
Solecki DJ, Gromeier M, Mueller S, Bernhardt G, Wimmer E. Expression of the human poliovirus receptor/CD155 gene is activated by sonic hedgehog. J Biol Chem. 2002 Jul 12;277(28):25697–25702.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 12, 2002

Volume

277

Issue

28

Start / End Page

25697 / 25702

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Trans-Activators
  • Receptors, Virus
  • Promoter Regions, Genetic
  • Mice, Transgenic
  • Mice, Inbred C3H
  • Mice
  • Membrane Proteins
  • Humans
  • Hedgehog Proteins