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Regulation of eukaryotic initiation factor 4E (eIF4E) phosphorylation by mitogen-activated protein kinase occurs through modulation of Mnk1-eIF4G interaction.

Publication ,  Journal Article
Shveygert, M; Kaiser, C; Bradrick, SS; Gromeier, M
Published in: Mol Cell Biol
November 2010

The m(7)G cap binding protein eukaryotic initiation factor 4E (eIF4E) is a rate-limiting determinant of protein synthesis. Elevated eIF4E levels, commonly associated with neoplasia, promote oncogenesis, and phosphorylation of eIF4E at Ser209 is critical for its tumorigenic potential. eIF4E phosphorylation is catalyzed by mitogen-activated protein kinase (MAPK)-interacting serine/threonine kinase (Mnk), a substrate of Erk1/2 and p38 MAPKs. Interaction with the scaffolding protein eIF4G, which also binds eIF4E, brings Mnk and its substrate into physical proximity. Thus, Mnk-eIF4G interaction is important for eIF4E phosphorylation. Through coimmunoprecipitation assays, we showed that MAPK-mediated phosphorylation of the Mnk1 active site controls eIF4G binding. Utilizing a naturally occurring splice variant, we demonstrated that the C-terminal domain of Mnk1 restricts its interaction with eIF4G, preventing eIF4E phosphorylation in the absence of MAPK signaling. Furthermore, using a small-molecule Mnk1 inhibitor and kinase-dead mutant, we established that Mnk1 autoregulates its interaction with eIF4G, releasing itself from the scaffold after phosphorylation of its substrate. Our findings indicate tight control of eIF4E phosphorylation through modulation of Mnk1-eIF4G interaction.

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Published In

Mol Cell Biol

DOI

EISSN

1098-5549

Publication Date

November 2010

Volume

30

Issue

21

Start / End Page

5160 / 5167

Location

United States

Related Subject Headings

  • Recombinant Proteins
  • Protein Serine-Threonine Kinases
  • Phosphorylation
  • Mice, Knockout
  • Mice
  • MAP Kinase Signaling System
  • Intracellular Signaling Peptides and Proteins
  • Humans
  • Extracellular Signal-Regulated MAP Kinases
  • Eukaryotic Initiation Factor-4G
 

Citation

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Shveygert, M., Kaiser, C., Bradrick, S. S., & Gromeier, M. (2010). Regulation of eukaryotic initiation factor 4E (eIF4E) phosphorylation by mitogen-activated protein kinase occurs through modulation of Mnk1-eIF4G interaction. Mol Cell Biol, 30(21), 5160–5167. https://doi.org/10.1128/MCB.00448-10
Shveygert, Mayya, Constanze Kaiser, Shelton S. Bradrick, and Matthias Gromeier. “Regulation of eukaryotic initiation factor 4E (eIF4E) phosphorylation by mitogen-activated protein kinase occurs through modulation of Mnk1-eIF4G interaction.Mol Cell Biol 30, no. 21 (November 2010): 5160–67. https://doi.org/10.1128/MCB.00448-10.
Shveygert, Mayya, et al. “Regulation of eukaryotic initiation factor 4E (eIF4E) phosphorylation by mitogen-activated protein kinase occurs through modulation of Mnk1-eIF4G interaction.Mol Cell Biol, vol. 30, no. 21, Nov. 2010, pp. 5160–67. Pubmed, doi:10.1128/MCB.00448-10.

Published In

Mol Cell Biol

DOI

EISSN

1098-5549

Publication Date

November 2010

Volume

30

Issue

21

Start / End Page

5160 / 5167

Location

United States

Related Subject Headings

  • Recombinant Proteins
  • Protein Serine-Threonine Kinases
  • Phosphorylation
  • Mice, Knockout
  • Mice
  • MAP Kinase Signaling System
  • Intracellular Signaling Peptides and Proteins
  • Humans
  • Extracellular Signal-Regulated MAP Kinases
  • Eukaryotic Initiation Factor-4G