Apolipoprotein E and mimetic peptide initiate a calcium-dependent signaling response in macrophages.

Published

Journal Article

Apolipoprotein E (ApoE) is a 34-kDa cholesterol transport protein that also possesses immunomodulatory properties. In this study, we demonstrate that ApoE initiates a signaling cascade in murine peritoneal macrophages that leads to increased production of inositol triphosphate with mobilization of intracellular Ca(2+) stores. This cascade is inhibited by pretreatment with receptor-associated protein and Ni(2+), and it is mediated by a pertussis toxin-sensitive G protein. These properties are characteristic of signal transduction induced via ligand binding to the cellular receptor, lipoprotein receptor-related protein. A peptide derived from the receptor-binding region of ApoE also initiates signal transduction in a manner similar to that of the intact protein, suggesting that this isolated region is sufficient for signal transduction. The ApoE-mimetic peptide competed for binding with the intact protein, confirming that they both interact with the same site. ApoE-dependent signal transduction might play a role in mediating the functional properties of this lipoprotein.

Full Text

Duke Authors

Cited Authors

  • Misra, UK; Adlakha, CL; Gawdi, G; McMillian, MK; Pizzo, SV; Laskowitz, DT

Published Date

  • October 1, 2001

Published In

Volume / Issue

  • 70 / 4

Start / End Page

  • 677 - 683

PubMed ID

  • 11590206

Pubmed Central ID

  • 11590206

Electronic International Standard Serial Number (EISSN)

  • 1938-3673

International Standard Serial Number (ISSN)

  • 0741-5400

Language

  • eng