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Interaction of plasminogen with dipeptidyl peptidase IV initiates a signal transduction mechanism which regulates expression of matrix metalloproteinase-9 by prostate cancer cells.

Publication ,  Journal Article
Gonzalez-Gronow, M; Grenett, HE; Weber, MR; Gawdi, G; Pizzo, SV
Published in: Biochem J
April 15, 2001

Both plasminogen (Pg) activation and matrix metalloproteinases (MMPs) are involved in the proteolytic degradation of extracellular matrix components, a requisite event for malignant cell metastasis. The highly invasive 1-LN human prostate tumour cell line synthesizes and secretes large amounts of Pg activators and MMPs. We demonstrate here that the Pg type 2 (Pg 2) receptor in these cells is composed primarily of the membrane glycoprotein dipeptidyl peptidase IV (DPP IV). Pg 2 has six glycoforms that differ in their sialic acid content. Only the highly sialylated Pg 2gamma, Pg 2delta and Pg 2epsilon glycoforms bind to DPP IV via their carbohydrate chains and induce a Ca(2+) signalling cascade; however, Pg 2epsilon alone is also able to significantly stimulate expression of MMP-9. We further demonstrate that the Pg-mediated invasive activity of 1-LN cells is dependent on the availability of Pg 2epsilon. This is the first demonstration of a direct association between the expression of MMP-9 and the Pg activation system.

Duke Scholars

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

April 15, 2001

Volume

355

Issue

Pt 2

Start / End Page

397 / 407

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Protein Binding
  • Prostatic Neoplasms
  • Plasminogen
  • Matrix Metalloproteinase 9
  • Male
  • Humans
  • Gene Expression Regulation
  • Flow Cytometry
  • Dipeptidyl Peptidase 4
 

Citation

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Gonzalez-Gronow, M., Grenett, H. E., Weber, M. R., Gawdi, G., & Pizzo, S. V. (2001). Interaction of plasminogen with dipeptidyl peptidase IV initiates a signal transduction mechanism which regulates expression of matrix metalloproteinase-9 by prostate cancer cells. Biochem J, 355(Pt 2), 397–407. https://doi.org/10.1042/0264-6021:3550397
Gonzalez-Gronow, M., H. E. Grenett, M. R. Weber, G. Gawdi, and S. V. Pizzo. “Interaction of plasminogen with dipeptidyl peptidase IV initiates a signal transduction mechanism which regulates expression of matrix metalloproteinase-9 by prostate cancer cells.Biochem J 355, no. Pt 2 (April 15, 2001): 397–407. https://doi.org/10.1042/0264-6021:3550397.
Gonzalez-Gronow, M., et al. “Interaction of plasminogen with dipeptidyl peptidase IV initiates a signal transduction mechanism which regulates expression of matrix metalloproteinase-9 by prostate cancer cells.Biochem J, vol. 355, no. Pt 2, Apr. 2001, pp. 397–407. Pubmed, doi:10.1042/0264-6021:3550397.
Journal cover image

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

April 15, 2001

Volume

355

Issue

Pt 2

Start / End Page

397 / 407

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Protein Binding
  • Prostatic Neoplasms
  • Plasminogen
  • Matrix Metalloproteinase 9
  • Male
  • Humans
  • Gene Expression Regulation
  • Flow Cytometry
  • Dipeptidyl Peptidase 4