Skip to main content
Journal cover image

A sphingosine kinase inhibitor induces cell death in temozolomide resistant glioblastoma cells.

Publication ,  Journal Article
Bektas, M; Johnson, SP; Poe, WE; Bigner, DD; Friedman, HS
Published in: Cancer Chemother Pharmacol
October 2009

PURPOSE: Sphingosine kinase is an oncogene that is up-regulated in several solid tumors. The product of the sphingosine kinase activity, sphingosine-1-phosphate is a potent mitogen involved in diverse cell processes such as cell survival and migration. Current standard therapy in the treatment of glioblastoma multiforme (GBM) is a combination of surgery, radiation, and chemotherapy using the drug temozolomide (TMZ). However, virtually all tumors become resistant to TMZ. Therefore, new drug targets are necessary. In this study, we investigated whether inhibiting sphingosine kinase could induce cell death in TMZ-resistant GBM cells. METHODS: To study TMZ resistance in vitro, we have generated TMZ-resistant cell lines from established GBM cells. We used a potent inhibitor of sphingosine kinase to study its effect on colony formation and cell growth in GBM cells with a limited dilution and WST assay. Moreover, cell death was determined by measuring caspase-3 activity using flow cytometry. RESULTS: A sphingosine kinase inhibitor reduced cell colony formation and activated caspase-3 in both TMZ-sensitive and resistant GBM cells. CONCLUSION: Addition of a sphingosine kinase inhibitor to the standard chemotherapy regimen against GBM may be beneficial.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cancer Chemother Pharmacol

DOI

EISSN

1432-0843

Publication Date

October 2009

Volume

64

Issue

5

Start / End Page

1053 / 1058

Location

Germany

Related Subject Headings

  • Thiazoles
  • Temozolomide
  • Reverse Transcriptase Polymerase Chain Reaction
  • Phosphotransferases (Alcohol Group Acceptor)
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Indicator Dilution Techniques
  • Humans
  • Glioblastoma
  • Flow Cytometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bektas, M., Johnson, S. P., Poe, W. E., Bigner, D. D., & Friedman, H. S. (2009). A sphingosine kinase inhibitor induces cell death in temozolomide resistant glioblastoma cells. Cancer Chemother Pharmacol, 64(5), 1053–1058. https://doi.org/10.1007/s00280-009-1063-0
Bektas, Meryem, Stewart P. Johnson, William E. Poe, Darell D. Bigner, and Henry S. Friedman. “A sphingosine kinase inhibitor induces cell death in temozolomide resistant glioblastoma cells.Cancer Chemother Pharmacol 64, no. 5 (October 2009): 1053–58. https://doi.org/10.1007/s00280-009-1063-0.
Bektas M, Johnson SP, Poe WE, Bigner DD, Friedman HS. A sphingosine kinase inhibitor induces cell death in temozolomide resistant glioblastoma cells. Cancer Chemother Pharmacol. 2009 Oct;64(5):1053–8.
Bektas, Meryem, et al. “A sphingosine kinase inhibitor induces cell death in temozolomide resistant glioblastoma cells.Cancer Chemother Pharmacol, vol. 64, no. 5, Oct. 2009, pp. 1053–58. Pubmed, doi:10.1007/s00280-009-1063-0.
Bektas M, Johnson SP, Poe WE, Bigner DD, Friedman HS. A sphingosine kinase inhibitor induces cell death in temozolomide resistant glioblastoma cells. Cancer Chemother Pharmacol. 2009 Oct;64(5):1053–1058.
Journal cover image

Published In

Cancer Chemother Pharmacol

DOI

EISSN

1432-0843

Publication Date

October 2009

Volume

64

Issue

5

Start / End Page

1053 / 1058

Location

Germany

Related Subject Headings

  • Thiazoles
  • Temozolomide
  • Reverse Transcriptase Polymerase Chain Reaction
  • Phosphotransferases (Alcohol Group Acceptor)
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Indicator Dilution Techniques
  • Humans
  • Glioblastoma
  • Flow Cytometry