TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.

Journal Article (Journal Article)

Uropathogenic Escherichia coli invade bladder epithelial cells (BECs) by direct entry into specialized cAMP regulated exocytic compartments. Remarkably, a significant number of these intracellular bacteria are subsequently expelled in a nonlytic and piecemeal fashion by infected BECs. Here, we report that expulsion of intracellular E. coli by infected BECs is initiated by the pattern recognition receptor, Toll-like receptor (TLR)4, after activation by LPS. Also, we reveal that caveolin-1, Rab27b, PKA, and MyRIP are components of the exocytic compartment, and that they form a complex involved in the exocytosis of bacteria. This capacity of TLR4 to mediate the expulsion of intracellular bacteria from infected cells represents a previously unrecognized function for this innate immune receptor.

Full Text

Duke Authors

Cited Authors

  • Song, J; Bishop, BL; Li, G; Grady, R; Stapleton, A; Abraham, SN

Published Date

  • September 1, 2009

Published In

Volume / Issue

  • 106 / 35

Start / End Page

  • 14966 - 14971

PubMed ID

  • 19706440

Pubmed Central ID

  • PMC2736405

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0900527106

Language

  • eng

Conference Location

  • United States