TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.


Journal Article

Uropathogenic Escherichia coli invade bladder epithelial cells (BECs) by direct entry into specialized cAMP regulated exocytic compartments. Remarkably, a significant number of these intracellular bacteria are subsequently expelled in a nonlytic and piecemeal fashion by infected BECs. Here, we report that expulsion of intracellular E. coli by infected BECs is initiated by the pattern recognition receptor, Toll-like receptor (TLR)4, after activation by LPS. Also, we reveal that caveolin-1, Rab27b, PKA, and MyRIP are components of the exocytic compartment, and that they form a complex involved in the exocytosis of bacteria. This capacity of TLR4 to mediate the expulsion of intracellular bacteria from infected cells represents a previously unrecognized function for this innate immune receptor.

Full Text

Duke Authors

Cited Authors

  • Song, J; Bishop, BL; Li, G; Grady, R; Stapleton, A; Abraham, SN

Published Date

  • September 1, 2009

Published In

Volume / Issue

  • 106 / 35

Start / End Page

  • 14966 - 14971

PubMed ID

  • 19706440

Pubmed Central ID

  • 19706440

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0900527106


  • eng

Conference Location

  • United States