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Detection of silica particles in lung tissue by polarizing light microscopy.

Publication ,  Journal Article
McDonald, JW; Roggli, VL
Published in: Arch Pathol Lab Med
March 1995

The detectability of silica particles in ordinary histologic sections by means of polarizing light microscopy has been controversial. Through the application of both correlative light microscopy and scanning electron microscopy to different lung sections, we showed that particles demonstrating peaks for silicon only by energy-dispersive x-ray microanalysis were in fact visible by polarizing light microscopy. These particles included some silica particles less than 1 microns in diameter. We found no correlation between the intensity level of the light source used for polarization and the differential ease of visualizing silica versus silicates; examples of both could be detected at both relatively low and high light intensities. This suggests that geometric parameters may be as important as composition in the ease of detection of silica versus silicates with polarized light.

Duke Scholars

Published In

Arch Pathol Lab Med

ISSN

0003-9985

Publication Date

March 1995

Volume

119

Issue

3

Start / End Page

242 / 246

Location

United States

Related Subject Headings

  • Silicosis
  • Silicon Dioxide
  • Pulmonary Fibrosis
  • Pathology
  • Microscopy, Polarization
  • Lung
  • Humans
  • Electron Probe Microanalysis
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McDonald, J. W., & Roggli, V. L. (1995). Detection of silica particles in lung tissue by polarizing light microscopy. Arch Pathol Lab Med, 119(3), 242–246.
McDonald, J. W., and V. L. Roggli. “Detection of silica particles in lung tissue by polarizing light microscopy.Arch Pathol Lab Med 119, no. 3 (March 1995): 242–46.
McDonald JW, Roggli VL. Detection of silica particles in lung tissue by polarizing light microscopy. Arch Pathol Lab Med. 1995 Mar;119(3):242–6.
McDonald, J. W., and V. L. Roggli. “Detection of silica particles in lung tissue by polarizing light microscopy.Arch Pathol Lab Med, vol. 119, no. 3, Mar. 1995, pp. 242–46.
McDonald JW, Roggli VL. Detection of silica particles in lung tissue by polarizing light microscopy. Arch Pathol Lab Med. 1995 Mar;119(3):242–246.

Published In

Arch Pathol Lab Med

ISSN

0003-9985

Publication Date

March 1995

Volume

119

Issue

3

Start / End Page

242 / 246

Location

United States

Related Subject Headings

  • Silicosis
  • Silicon Dioxide
  • Pulmonary Fibrosis
  • Pathology
  • Microscopy, Polarization
  • Lung
  • Humans
  • Electron Probe Microanalysis
  • 3202 Clinical sciences
  • 1103 Clinical Sciences