Assessment of similarity among coagulase-negative staphylococci from sequential blood cultures of neonates and children by pulsed-field gel electrophoresis.

Journal Article (Journal Article)

One of the criteria used to determine the clinical importance of coagulase-negative staphylococci (CoNS) is isolation of the bacteria from sequential blood cultures. Pulsed-field gel electrophoresis was used to characterize sequential blood isolates of CoNS collected within a 7-day period from neonates and children with bacteremia. Of 18 episodes among neonates, 6 (33%) involved unrelated strains of CoNS. All unrelated strains were from neonates who received antimicrobial therapy after the first culture and who had a second culture > or = 36 h later. Among older children, 5 (19%) of the 27 episodes of presumed central venous (CV) catheter-related sepsis involved unrelated isolates. All of the unrelated isolates were from patients who had blood samples obtained through CV catheters only. Thus, even repeated isolation of CoNS from blood cultures may represent contamination if samples are drawn through CV catheters only or if second samples are obtained > 1 day after appropriate antimicrobial therapy.

Full Text

Duke Authors

Cited Authors

  • Zaidi, AK; Harrell, LJ; Rost, JR; Reller, LB

Published Date

  • November 1996

Published In

Volume / Issue

  • 174 / 5

Start / End Page

  • 1010 - 1014

PubMed ID

  • 8896502

International Standard Serial Number (ISSN)

  • 0022-1899

Digital Object Identifier (DOI)

  • 10.1093/infdis/174.5.1010


  • eng

Conference Location

  • United States