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Bioluminescence screening for bacteriuria.

Publication ,  Journal Article
Kolbeck, JC; Padgett, RA; Estevez, EG; Harrell, LJ
Published in: J Clin Microbiol
April 1985

A rapid screening test (45 min) for bacteriuria was evaluated in 1,000 clinical urine specimens. The test procedure is based upon firefly luciferase analysis of bacterial ATP and uses the Lumac kit and Lumac M2010 Biocounter (3M Co., St. Paul, Minn.). The procedure allows for removal and destruction of nonbacterial ATP and subsequent analysis of bacterial ATP by firefly luciferase with a single photon counter. Results, expressed in relative light units, were compared with actual CFU by the calibrated loop technique. Sensitivities and specificities were calculated separately for clean-catch midstream specimens and for urines obtained by catheterization. The sensitivity for 719 clean-catch specimens with a Lumac cutoff of greater than or equal to 500 relative light units, representing greater than or equal to 10(5) CFU/ml, was 93%. The sensitivity for 281 catheterized specimens with a Lumac cutoff of greater than or equal to 200 relative light units, representing greater than or equal to 10(4) CFU/ml, was 95%. There were 19 false-negative results in the 1,000 specimens tested; more than 50% of these were contaminated cultures and were not considered significant in determining bacteriuria. In conclusion, the Lumac bioluminescence assay is a reliable, rapid bacteriuria screening technique with the potential of reducing the laboratory cost and for reducing the turnaround time in processing negative urine cultures.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1985

Volume

21

Issue

4

Start / End Page

527 / 530

Location

United States

Related Subject Headings

  • Microbiology
  • Mathematics
  • Luminescent Measurements
  • Humans
  • False Negative Reactions
  • Costs and Cost Analysis
  • Bacteriuria
  • Bacteriological Techniques
  • 3207 Medical microbiology
  • 3202 Clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Kolbeck, J. C., Padgett, R. A., Estevez, E. G., & Harrell, L. J. (1985). Bioluminescence screening for bacteriuria. J Clin Microbiol, 21(4), 527–530. https://doi.org/10.1128/jcm.21.4.527-530.1985
Kolbeck, J. C., R. A. Padgett, E. G. Estevez, and L. J. Harrell. “Bioluminescence screening for bacteriuria.J Clin Microbiol 21, no. 4 (April 1985): 527–30. https://doi.org/10.1128/jcm.21.4.527-530.1985.
Kolbeck JC, Padgett RA, Estevez EG, Harrell LJ. Bioluminescence screening for bacteriuria. J Clin Microbiol. 1985 Apr;21(4):527–30.
Kolbeck, J. C., et al. “Bioluminescence screening for bacteriuria.J Clin Microbiol, vol. 21, no. 4, Apr. 1985, pp. 527–30. Pubmed, doi:10.1128/jcm.21.4.527-530.1985.
Kolbeck JC, Padgett RA, Estevez EG, Harrell LJ. Bioluminescence screening for bacteriuria. J Clin Microbiol. 1985 Apr;21(4):527–530.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1985

Volume

21

Issue

4

Start / End Page

527 / 530

Location

United States

Related Subject Headings

  • Microbiology
  • Mathematics
  • Luminescent Measurements
  • Humans
  • False Negative Reactions
  • Costs and Cost Analysis
  • Bacteriuria
  • Bacteriological Techniques
  • 3207 Medical microbiology
  • 3202 Clinical sciences