Stimulation of lymphocyte responses by angiotensin II promotes kidney injury in hypertension.

Published

Journal Article

Activation of the renin-angiotensin system contributes to the progression of chronic kidney disease. Based on the known cellular effects of ANG II to promote inflammation, we posited that stimulation of lymphocyte responses by ANG II might contribute to the pathogenesis of hypertensive kidney injury. We therefore examined the effects of the immunosuppressive agent mycophenolate mofetil (MMF) on the course of hypertension and kidney disease induced by chronic infusion of ANG II in 129/SvEv mice. Although it had no effect on the severity of hypertension or cardiac hypertrophy, treatment with MMF significantly reduced albuminuria and ameliorated kidney injury, decreasing glomerulosclerosis and reducing lymphocyte infiltration into the renal interstitium. Attenuation of renal pathology with MMF was associated with reduced expression of mRNAs for the proinflammatory cytokines interferon-gamma and tumor necrosis factor-alpha and the profibrotic cytokine transforming growth factor-beta. As infiltration of the kidney by T lymphocytes was a prominent feature of ANG II-dependent renal injury, we carried out experiments examining the effects of ANG II on lymphocytes in vitro. We find that exposure of splenic lymphocytes to ANG II causes prominent rearrangements of the actin cytoskeleton. These actions require the activity of Rho kinase. Thus, ANG II exaggerates hypertensive kidney injury by stimulating lymphocyte responses. These proinflammatory actions of ANG II seem to have a proclivity for inducing kidney injury while having negligible actions in the pathogenesis of cardiac hypertrophy.

Full Text

Duke Authors

Cited Authors

  • Crowley, SD; Frey, CW; Gould, SK; Griffiths, R; Ruiz, P; Burchette, JL; Howell, DN; Makhanova, N; Yan, M; Kim, H-S; Tharaux, P-L; Coffman, TM

Published Date

  • August 2008

Published In

Volume / Issue

  • 295 / 2

Start / End Page

  • F515 - F524

PubMed ID

  • 18495795

Pubmed Central ID

  • 18495795

Electronic International Standard Serial Number (EISSN)

  • 1522-1466

International Standard Serial Number (ISSN)

  • 1931-857X

Digital Object Identifier (DOI)

  • 10.1152/ajprenal.00527.2007

Language

  • eng